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Bioscience
Published by Universitas Negeri Padang
ISSN : 2614669X     EISSN : 2579308X     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Bioscience ISSN 2579-308X (Electronic) ISSN: 2614-669X (Print) is peer-reviewed journal and scientific journal publish by Universitas Negeri Padang. The aim of this journal is to publish articles dedicated to all aspects of the latest outstanding developments in the field of biology. Scope of this journal is ;Environmental Biology; Genetics and Biotechnology; Biology of Function; Systematics, Structure and Development.
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Articles 9 Documents
 1 
Search results for , issue "Vol 5, No 1 (2021): Biology" : 9 Documents clear
The Effect of Coffee Arabica (Coffea arabica L.) Fruit Skin Extracts on Small Intestine Morphometry of mice (Mus musculus L.) with Ethanol-Induced Rita Maliza; Febriofca Galih Yatalaththov; Haris Setiawan; Listiatie Budi Utami
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111571-0-00

Abstract

Consumption of alcoholic beverages can increase ROS and inflammation. In the duodenal, the consumption of alcohol can result in erosions, bleeding, and mucosal injury. Arabica coffee fruit skin contains antioxidant substances, which are expected to have a good role as an antioxidant effect. Currently, unknown effects of alcohol and extract of Arabica coffee fruit skin administration on the damage of the small intestine of mice (Mus musculus L.). This study used an experimental design, and 25 males of mice were divided into five groups of treatment for 15 days. After the treatment, the mice at necropsied and the small intestine were taken for histopathological examination using hematoxyline-eosin staining. The Data analyzed used One Way ANOVA and Duncan's tests. Histopathological observations showed a dose of 250 mg/kg BW experienced the most significant improvement damage of the small intestine of mice compared to other treatments. Histomorphometry measurement, The dose of 250 mg/kg BB showed the high of villi 272,10 ± 13,36 µm, (P<0.05), duodenal villi crypt depth 117,33 ± 30,35 µm (P<0.05) and surface area of small intestinal villi 7,66 ± 0,55 mm2 (P<0.05) was similar with negative control. The conclusion of this study is a dose of 250 mg/kg BW treatment was able to improve the histopathology and morphometry small intestine of mice that were damaged due to consumption of ethanol 15%.
Analysis of Genetic Variations in Poly Gene Sequences in Dengue Virus 2 Using In-Silico RFLP Afifatul Achyar; Alvenaya Hindayageni; Fadhila Humaira; Nurfadillatun Nisa Wijaya; Nur Aqsha; Zultsatunni’mah Zultsatunni’mah
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111916-0-00

Abstract

Dengue hemorrhagic fever (DHF) is a disease caused by Dengue virus  transmitted to humans through infected Aedes aegypti and Ae. Albopictus. Dengue virus classified into 4 serotypes, including DEN-1, DEN-2, DEN-3, and DEN-4. The genomes of the four dengue viruses share about 65% similarities, the rest is a genetic variation that differentiates between serotypes. Genetic variations also occur even within one serotype. This study aimed to analyse genetic variation in envelope protein E (POLY) gene sequences in Dengue Virus 2 NCBI PopSet 1760494694 using in-silico RFLP by free bioinformatic tools on internet. The restriction enzymes used were AfeI and ApaLI. The results of the in-silico RFLP in this study showed that there were genetic variations in the recognition site of the AfeI enzymes (A1 allele and A2 allele) and ApaLI (B1 allele and B2 allele) in 18 DNA sequences of the POLY gene for Dengue 2 virus NCBI PopSet 1760494694.
The Sub Acute Toxicity Study of Purified Gambir (Uncaria gambir Roxb.) to Liver Histology and its Reversibility on Rats Dita Permatasari; Indri Oktavia; Armenia Nazar; Almahdy Ahmadin
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111642-0-00

Abstract

Purified gambir proved to have many pharmacological activities and has the potential to be developed into standardized herbal medicines. This study was conducted to determine the safety of purified gambir to the liver histology. A total of 30 female white rats were used which divided into 4 groups: one control group and 3 test groups treated with purified gambir at doses of 5, 10 and 20 mg/kgbw orally for 14 days respectively. On the scheduled days, three animals from each group were killed for liver histological examination. The degree of liver damage and liver organ ratio in this study where were measured. The reversibility of liver damage was observed from the change in the value of the damage degree and liver organ ratio after the administration of gambir was stopped. Data were analyzed using two-way ANOVA followed by Duncan (significance were taken at p<0.05). The administration of purified gambir in rats for 14 days at tested groups caused significant damage to liver tissue (p<0.05). In this situation, the degree of tissue damage increased and the liver organ ratio decreased in all tested groups. After the administration was stopped, the degree of liver damage is decreased insignificantly, meanwhile the liver organ ratio is significantly increased.
Study of Forest Types, Inventory of Tree, and Chlorofil Contents of Malabar Forest Leaves, Malang City Roimil Latifa; Endrik Nurrohman; Samsun hadi
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111466-0-00

Abstract

This research aims to examine the forest types, inventory of tree species, and chlorophyll content of plant leaves in the Malabar forest, Malang City. This type of research is descriptive quantitative. This research was done from the month of August to December 2020 and took place in Malabar Forest and Biology Laboratory of University of Muhammadiyah Malang. Data collection methods using exploratory techniques and laboratory observations. Data analysis is done by descriptive quantitative. The results showed that 101 tree species inhabit Malabar forest with the most Tanjung trees with 175, and the least number is Srikaya with only one tree. Malabar forest is classified as a forest with a spreading form. In the dry season, the average chlorophyll ‘a’ content of leaves was highest in starfruit leaves (35.848 µg/ml), the lowest average of starfruit leaves (17.857µg/ml), the average chlorophyll ‘b’ content of leaves was highest in Tabebuya leaves (58.862µg/ml). The lowest was Norfolk Pine leaf (9,124 µg/ml), the highest total leaf chlorophyll was Tabebuya leaf (91,737µg/ml), and the lowest was the Norfolk Pine leaf (28,517µg/ml). In the rainy season, the highest chlorophyll ‘a’ content was Sengon tree (34.3µg/ml) and the lowest was Chocolate (0.3µg/ml), the highest chlorophyll ‘b’ was Genitu (131.6µg/ml) the lowest was Lamtoro (6.5µg/ml), the highest total chlorophyll was Melinjo (90.7µg/ml) and the lowest was Kol Banda (3.3µg/ml). Keywords: Malabar Forest, Inventory, Leaf Chlorophyll.
Serum Protein Total Determination After Hypoxic Swimming Exercises in Trained and Untrained Swimmers Using The Nanophotometric Method Siska Alicia Farma; Syahrastani Syahrastani
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111936-0-00

Abstract

Anaerobic physical exercise aims to increase the explosive power, which is the ability of a person's muscles to do work with maximum strength in the fastest possible time. Sprint swimming exercises are often called hypoxic swimming exercises. Protein biomolecules have a major function in building muscle mass, maintaining body defences and increasing sports performance. Physical exercise occurs in a variety of systemic and cellular responses involving metabolic, immunological and hormonal changes. It is necessary to know how the total protein profile in serum after physical exercise in this case was limited to a single hypoxic swimming exercise. This was experimental research with post-test only control group design. There were three training groups, namely (5) control, (5) trained, and (5) untrained based on inclusion and exclusion criteria with the purposive sampling method. Single hypoxic swimming exercise (sprint) did along 200 m. The swimming training pattern was per 50 m without resting with two types of styles swim, freestyle and breaststroke. Measurement of total protein levels was carried out using a nanophotometer where the volume of serum used was very small. The results showed that the total protein content between the control group and the treatment group was significantly different (ANOVA, p <0.05). Between the trained and untrained groups, a decrease in total protein levels was seen in the trained group. This is probably due to the function of these proteins. It can be concluded that protein is more widely used by the body as a building element in the body, especially muscle mass. Cellular adaptive response is better when the body is in a trained condition.
Vivivary Tests on Mangrove Plants Species Rhizophora apiculata and Avicennia alba Against the Level of Salt Salinity and Tide Pandu Prabowo Warsodirejo; Tika Santika; RA Rina Dian Anggraini; Widya Sari
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151110894-0-00

Abstract

Indonesia's coastal areas have a large area and potential for mangrove ecosystems. Of the approximately 15,900 million ha of mangrove forests in the world. 27% or 4,293 million ha are in Indonesia. The Sicanang Medan Belawan mangrove forest, North Sumatra Province, is a wetland area which mostly consists of mangrove forests. In the mangrove forest, there are Rhizophora apiculata and Avicennia alba species. Mangroves have unique morphological characters as a form of adaptation to the environment, one of which is the salinity of sea water which is the level of salinity or salt content in seawater. In the mangrove forest of Sicanang Medan Belawan, there are Rhizophora apiculata and Avicennia alba species which have the characteristics of vivivar culture development. From the results of test 1 on the species Rhizopora apiculata, it was found that the highest salinity level at the muddy and sandy station was 30 ppm, and the highest pH was at 7.79, and at a temperature of 29 C. While for Avicennia alba the highest salinity value was obtained at 30.6 ppm, the highest pH was at 7.82. , and temperature at 29.5 C. Based on the results that different zoning could affect yield, the vivivary shape of mangroves also had an effect on yield. salinity affects mangroves to develop, the higher the salinity, the more difficult it is for the seeds to develop
Diversity of Medicinal Plant and Potential as Antibacterial in Peret Hill Forest, Tiang Tarah Village, District of Bakam, West Bangka Monica Kharisma Swandi; Neli Sulastri; Fitri Ulandari; Mega Febriyanti
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151111785-0-00

Abstract

Traditional medicine that has been done by the Tiang Tarah Village shaman, Bakam District, Bangka Regency uses plants as medicine to cure various diseases. However, so far some plants that are used as medicine can not be proven definitively effectiveness against a disease. Therefore, this research was conducted with the aim of knowing which plants are used as traditional medicine in Tiang Tarah Village, Bakam District, Bangka Regency, exploring the diversity of medicinal plants in the Bukit Peret Forest, and testing the antibacterial activity of medicinal plants against Escherichia coli and Staphylococcus aureus bacteria. The research was conducted with several stages, namely: (i) Interviewing informants who are experienced in the field of traditional medicine (shaman) using snowball sampling techniques, (ii) Exploration in the Peret Hill Forest for sampling using roaming method, (iii) Antibacterial activity test medicinal plants against E. coli and S. aureus by using agar diffusion method. The results of the study obtained 22 types of medicinal plants interviewed by 3 shamans Tiang Tarah Village. Of the 22 species, only 14 were found during exploration in the Peret Hill Forest. 10 of them have antibacterial activity against E. coli and 11 types against S.aureus.
Isolation and Identification of Probiotic Candidate Lactic Acid Bacteria (Lab) from Shrimp Paste (Mysis relicta) Based on 16s rRNA Gene Rahmat Wahyudi Putra; Resti Fevria
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151107569-0-00

Abstract

Terasi is one of the fermented product of shrimp or fish which is often used as food seasoning by the people. Terasi is also a source of Lactic Acid Bacteria (LAB) which has the potential as a culture for probiotic starters. Probiotics are dietary supplements containing live microbes that profitable to their hosts. This study aimed to identify probiotic lactic acid bacteria isolated from terasi of shrimp based on the 16S ribosomal ribonucleic acid (rRNA) gene sequence. LAB isolation from terasi was done by pour plate method using selective medium, MRSA (Man Rogosa Sharpe Agar). The identification of LAB in this study used a method of molecular with a marker gene of 16S rRNA. A total of 3 isolates were obtained from the isolation process. All LAB isolates are rod (Basil) positive Gram bacteria, negative catalase. Two isolates (TR 1 and TR 2) were identified as Enterococcus faecalis. While one more isolate (TR 3) were identified as Weissella cibaria.
Anti-Microbial Activities of Shallots (Allium cepa L.) Extract and Garlic (Allium sativum L.) Extract on the Growth of Peat Soil Bacteria Chaidir Adam
Bioscience Vol 5, No 1 (2021): Biology
Publisher : UNIVERSITAS NEGERI PADANG

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/0202151110068-0-00

Abstract

This study aims to examine the antimicrobial activity of red onion (Allium cepa L.) and garlic (Allium sativum L.) extracts on the growth of peat soil bacteria. This type of research is an experimental study using a Completely Randomized Design with four levels of treatment. Test for bacterial activity using the agar diffusion method with the paper disc technique. Data were analyzed statistically using One Way Anova and the Games-Howell post-hoc test at the 5% significance level. The results showed that red onion (Allium cepa L.) and garlic (Allium sativum L.) extracts had antimicrobial activity against the growth of tested bacteria as indicated by the presence of a clear zone as an indicator of inhibition of bacterial growth. Garlic has better antimicrobial activity seen from the diameter of the clear zone that appears in the garlic extract treatment ranging from 8-13 mm, whereas in the treatment of onions it ranges from 3–5 mm. 

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